Initial Aflp Tagging of the Gene (cl) for Circumlineated Pattern

Introduction The circumlineatus gene (C/) was discovered by R. Prakken (1972). Circumlineatus is recessive and is expressed in partly colored seed coats (0 with restricted patterns, such as virgarcus. Cl was discovered in a cross between 'White J' {Tz cl Rk) x 'Soldaat K' (/ z Cl rlâ) and is denoted by a ''precipitation line" (Prakken, 1972) demarcating the boundaiy between the white and colored zones of the seed coat. Bassett (2004) further clarified the anatomy of the demarcation, by describing the precipitation line as a physical groove in the seed surface between seed coat colors in various genetic backgrounds, including mineral brown (v BC35-593), yellow brown {b v 5593), and shamois {gbv BC3 5-593). He found that only the black ( 5 F) F2 seed from each backcross did not present the cl phenotype, but that non-black virgarcus types were true breeding.